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TB Notes 3, 2002

Update from the Laboratory

Results of National Genotyping Network Project

The National Tuberculosis Genotyping and Surveillance Network research project is ending this year. The study enrollment period ran from 1996 through 2000, and data on 10,883 patients were included in the final databases. This represents about 11.6% of all TB cases in the United States during that period. A special issue of CDC’s Emerging Infectious Diseases planned for this fall will be devoted to reports from network participants. In addition to providing service for the research study, the regional genotyping laboratories have fingerprinted isolates submitted by TB control programs in their region in support of outbreak investigations and other studies. To continue this service, the National Center for Infectious Diseases (NCID) is planning to fund genotyping activities in regional laboratories via contracts; for that purpose, a request for proposals is being issued in late fall. This contract will be open to all entities in the United States, including public health laboratories, universities, research institutions, and commercial laboratories.

The results of the network study clearly demonstrate the utility of universal genotyping. However, the study also showed that standard fingerprinting by the IS6110 RFLP method is laborious and costly and the turnaround time is too long for efficient use by TB control programs. To overcome these difficulties, regional laboratories will use high-throughput, PCR-based genotyping methods to provide rapid results. The more specific IS6110 method will be reserved for typing isolates clustered by the PCR assays. The goal will be to provide genotyping for all M. tuberculosis isolates in the United States. This will require considerable commitment from the TB control programs, not only to acquire the isolates for typing, but also to integrate the results of genotyping into their program including contact investigations, outbreak studies, and program evaluation.

—Reported by Jack Crawford, PhD
Division of AIDS, STD, and TB Laboratory Research


Released October 2008
Centers for Disease Control and Prevention
National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention
Division of Tuberculosis Elimination -

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